Study on Esculetin to reduce cardiotoxicity induced by Doxorubicin

Authors

  • Xiao Li Department of Oncology, Affiliated Hospital of Chengde Medical College, 36 Nanyingzi Street, Shuangqiao, Chengde, Hebei 067000, P.R. China
  • Xiaolei Yu Department of Oncology, Affiliated Hospital of Chengde Medical College, P.R. China
  • Fan Xu Department of Radiology, Affiliated Hospital of Chengde Medical College, P.R. China
  • Qingshan Li Department of Radiology, Affiliated Hospital of Chengde Medical College, P.R. China
  • Xiao Xu Department of Pharmacy, Affiliated Hospital of Chengde Medical College, P.R. China

DOI:

https://doi.org/10.3126/njc.v5i1.41329

Keywords:

doxorubicin, esculetin, Apoptosis

Abstract

Objective: To investigate the protective effects and mechanisms of esculetin(Esc) on H9C2 cells injury induced by doxorubicin (DOX). 

Methods: H9C2 cells were cultured and divided into control group, model (DOX) group and intervention (ESC + DOX) group. Flow cytometry was used to detect apoptosis of H9C2 cells and reactive oxygen (ROS) level in H9C2 cells; Western blotting to detect the cleaved Caspase-3, cleaved PARP, bid and Bcl-2 protein expression in H9C2 cells. 

Results: The number of apoptosis and ROS level of H9C2 cells in the model group, the expression of cleaved Caspase-3, cleaved PARP and Bid protein in the model group were obviously higher than control group; and the Bcl-2 protein expression was obviously lower (P < 0.05).The number of apoptosis and ROS level, cleaved Caspase-3, cleaved PARP and Bid protein expression in H9C2 cells in intervention group were obviously lower than model group;the Bcl-2 protein expression was obviously higher (P< 0.05). 

Conclusion: Esculetin can reduce the cardiotoxicity induced by doxorubicin by reducing apoptosis and ROS level. 

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Published

2021-09-26

How to Cite

Li, X., Yu, X., Xu, F., Li, Q., & Xu, X. (2021). Study on Esculetin to reduce cardiotoxicity induced by Doxorubicin. Nepalese Journal of Cancer, 5(1), 70–73. https://doi.org/10.3126/njc.v5i1.41329

Issue

Vol. 5 No. 1 (2021)

Section

Original Articles

License

Copyright (c) 2021 Nepalese Journal of Cancer

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