GC-MS analysis, antibacterial, antioxidant study and brine shrimp lethality analysis of Trachyspermum ammi (L.) Sprague

Authors

  • Shova D.C. Amrit Campus, Tribhuvan University, Kathmandu, Nepal
  • Binita Maharjan Amrit Campus, Tribhuvan University, Kathmandu, Nepal
  • Timila Shrestha Amrit Campus, Tribhuvan University, Kathmandu, Nepal
  • Samjhana Bharati Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal
  • Ram Lal Shrestha Amrit Campus, Tribhuvan University, Kathmandu, Nepal

DOI:

https://doi.org/10.3126/arj.v1i1.32452

Keywords:

Trachyspermum ammi, Essential oil, GC-MS, Bio-activity

Abstract

Fruit part of Trachyspermum ammi (L.) Sprague was subjected to extraction of essential oil by hydrodistillation in Clevenger apparatus. So collected essential oil was analyzed by GC-MS for its composition and exhibited the presence of 10 different compounds. The most abundant were γ-terpinene (53.81%) and thymol (29.40%). Antibacterial activity was performed against six bacterial species and Staphylococcus aureus, Enterobacter cloaceae and Bacillus subtilis were the most susceptible to the essential oil showing zone of inhibition (ZOI) 1.4, 1.5 and 1.4 cm respectively. The IC50 value of the oil against DPPH was found to be 0.94 mg/mL. The LC50 value of essential oil of T. ammi against brine shrimp was found 26.2 μg/mL.

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Author Biographies

Shova D.C., Amrit Campus, Tribhuvan University, Kathmandu, Nepal

Department of Chemistry

Binita Maharjan, Amrit Campus, Tribhuvan University, Kathmandu, Nepal

Department of Chemistry

Timila Shrestha, Amrit Campus, Tribhuvan University, Kathmandu, Nepal

Department of Chemistry

Samjhana Bharati, Tri-Chandra Multiple Campus, Tribhuvan University, Kathmandu, Nepal

Department of Chemistry

Ram Lal Shrestha, Amrit Campus, Tribhuvan University, Kathmandu, Nepal

Department of Chemistry

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Published

2020-09-17

How to Cite

D.C., S., Maharjan, B., Shrestha, T., Bharati, S., & Shrestha, R. L. (2020). GC-MS analysis, antibacterial, antioxidant study and brine shrimp lethality analysis of Trachyspermum ammi (L.) Sprague. Amrit Research Journal, 1(1), 45–50. https://doi.org/10.3126/arj.v1i1.32452

Issue

Vol. 1 No. 1 (2020)

Section

Articles