In-vitro antibacterial activity of Rhus javanica against multidrug-resistant uro-pathogens
DOI:
https://doi.org/10.3126/jmcjms.v7i1.30005Keywords:
Rhus javanica, Escherichia coli, antibacterial, multidrug-resistantAbstract
Background and Objectives: Antibiotic resistance is emerging as a major public health concern. From the ancient time in history, different plants and herbs have been known to have medicinal value. Rhus javanica has been found to show antibacterial activity against pathogenic bacteria. Thus, present study is designed to investigate the prevalence of urinary tract infection causing bacterial pathogens, its antibiogram and effect of Rhus javanica in standard in-vitro condition against multidrug-resistant.
Material and Methods: Cross-sectional study was conducted in urinary tract infection suspected patients visiting a clinic in Kalimati, Kathmandu, from July to December 2017. A total of 133 midstream urine samples were collected and cultured in MacConkey agar and Blood agar media and isolates were identified by standard biochemical tests. Antibiotic susceptibility testing was performed according to Clinical and Laboratory Standard Institute (CLSI) guideline. Leaves extract of Rhus javanica was obtained by maceration using 50.0% methanol and susceptibility testing was performed by using cork borer method in Mueller-Hinton agar.
Results: Out of total 133 samples, 35.3%showed significant bacterial growth (i.e. 105 cfu/ml) on agar plate. The most predominant organism was Escherichia coli 17.3%, followed by Enterococcus faecalis 6.8%, Klebsiella pneumoniae 3.8%, Pseudomonas aeruginosa 3.0%, Proteus vulgaris 2.3%, and Staphylococcus aureus 2.3%. Out of 47 isolates, 53.2% were Multi Drug Resistant (MDR). Various concentrations of the crude extract used (25, 50, 75 and 100 mg/ml) showed susceptibility to all the bacterial pathogens isolated, with the diameter of zone of inhibition ranging from 12 to 28 mm.
Conclusion: Methanolic extract of Rhus javanica showed antibacterial activity against multidrug-resistant isolates of bacterial uropathogens in standard in-vitro condition.
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References
2. Centers for Disease Control and Prevention (CDC). Antibiotic / Antimicrobial Resistance (AR / AMR);2019. https://www.cdc.gov/drugresistance/about.html
3. Adhikari S, Saud B, Paudel G, Bajracharya D. Emergence of Antimicrobial Drug Resistant Bacteria in Nepal: A Current Scenario. Proteomics Bioinformatics Current Res, 2019; 1(1): 31-33.
https://doi.org/10.1155/2019/7960268
4. Shakya P, Shrestha D, Maharjan E, Sharma VK, Paudyal R.ESBL production among E. coli and Klebsiella spp. causing urinary tract infection: A hospital based study. Open Microbiol J 2017;11: 23-30.
https://doi.org/10.2174/1874285801711010023
5. Ansari S, Nepal HP, Gautam R et al. Childhood septicemia in Nepal: Documenting the bacterial etiology and its susceptibility to antibiotics. Int J Microbiol 2014: 1-6
https://doi.org/10.1155/2014/452648
6. Saud B, Malla R, Shrestha K. A Review on the Effect of Plant Extract on Mesenchymal Stem Cell Proliferation and Differentiation. Stem Cells International. 2019;2019.
https://doi.org/10.1155/2019/7513404
7. Kunwar RM. Ethnobotanical notes on flora of Khaptad National Park (KNP), far-western Nepal. Himalayan Journal of Sciences. 2003;1(1):25-30.
https://doi.org/10.3126/hjs.v1i1.182
8. Shan B, Cai YZ, Brooks JD, Corke H. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. International Journal of food microbiology. 2007;117(1):112-9.
https://doi.org/10.1016/j.ijfoodmicro.2007.03.003
9. Choi JG, Kang OH, Lee YSet.al. In vitro and in vivo antibacterial activity of Punica granatum peel ethanol extract against Salmonella. Evidence-Based Complementary and Alternative Medicine. 2011;2011.
https://doi.org/10.1093/ecam/nep105
10. You YO, Choi NY, Kang SY, Kim KJ. Antibacterial activity of Rhus javanica against methicillin-resistant Staphylococcus aureus. Evidence-based complementary and alternative medicine. 2013;2013.
https://doi.org/10.1155/2013/549207
11. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing 27th ed. CLSI supplement 2017: M100: P. A. Wayne, https://clsi.org/media/1469/m100s27_sample.pdf .
12. Chaudhari BK, Singh GK, Parajuli KP, Shrestha K. Incidence and susceptibility of uropathogens isolated among the patients at tertiary care hospital in Eastern Nepal. Journal of Nobel Medical College. 2016;5(2):51-5..
https://doi.org/10.3126/jonmc.v5i2.16318
13. Chaudhary R, Thapa SK, Rana JC, Shah PK. Surgical site infections and antimicrobial resistance pattern. J Nepal Health Res Counc 2017;15: 120-123.
https://doi.org/10.3126/jnhrc.v15i2.18185
14. Yang SK, Kim HJ, Oh SW. Antimicrobial Activities of Ethanol Extracts of R hus javanica and T erminalia chebula Against S higella Species. Journal of Food Safety. 2014;34(1):95-102.
https://doi.org/10.1111/jfs.12102
15. I. S. L. Im Seon Lee, S.-R. Oh, K.-S. Ahn, H.-K. Lee, "Semialactone, isofouquierone peroxide and fouquierone, three new dammarane triterpenes from Rhus javanica," Chemical and Pharmaceutical Bulletin 2001;49(8):1024-1026.
https://doi.org/10.1248/cpb.49.1024
16. Choi I. Antimicrobial activity of Rhus javanica extracts against animal husbandry disease-related bacteria. Journal of the Korean Society of Food Science and Nutrition. 2003;32(8):1214-20.
https://doi.org/10.3746/jkfn.2003.32.8.1214
17. Kim KH, Kim AR, Cho EJ et al. Antibacterial activity of Rhus javanica against the Fish Pathogens Vibrio ichthyoenteri and
Streptococcus iniae. Korean Journal of Fisheries and Aquatic Sciences. 2014;47(1):18-22.
https://doi.org/10.5657/KFAS.2014.0018
18. Cha B, Lee S, Rhim T, Lee K. Constituents of antioxidative activity and free radical scavenging effect from Galla Rhois (Rhus javanica Linne). Korean Journal of Pharmacognosy. 2000;31(2):185-9.
19. Saud B, Paudel G, Khichaju S et al. Multidrug-Resistant Bacteria from Raw Meat of Buffalo and Chicken, Nepal. Veterinary medicine international. 2019;2019.
https://doi.org/10.1155/2019/7960268
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