Assessment of liver function, bile secretory rate and liver histological changes in type 1 diabetes mellitus rats treated with ocimum gratissimum

Authors

  • Okon Akpan Uduak Department of Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Uyo, Uyo
  • Peter Imo Aniekan Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Uyo, Uyo
  • Esther Olusola Aluko Department of Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Uyo, Uyo

DOI:

https://doi.org/10.3126/ajms.v6i3.9960

Keywords:

Bile secretion, liver function test, liver histology, Ocimum gratissimum, Type 1 Diabetes mellitus

Abstract

Background: The liver plays an important role in nutrient metabolism and alterations in the liver function, biliary secretion and cytoachitecture are common in diabetes mellitus (DM).

Aim: Ocimum gratissimum (OG) has been reported to exert hypoglycaemic effect in DM, the need to assess the impact of its treatment on these diabetic complications became imperative.

Material and Methods: Phytochemical and toxicity test were conducted. Eighteen rats into three groups of six rats each were used. Group 1 was the control and given normal feed only. Group 2 was diabetic untreated rats (DM) while group 3 was OG treated diabetic rats (DMT) at a dose of 1500 mg/kg. All groups had access to food and water ad libitum. After 28 days, serum was obtained for analysis of Alkaline phosphatase (ALP), Alanine aminotransferase (ALT) and Aspartate aminotranferase (AST). The bile content was collected at 3 hours interval to determine the bile secretory rate. Routine biopsy method was employed for histological studies.

Results: Results showed that AST in the DM and DMT groups were significantly higher (P < 0.001) than the control group; DM was significantly higher (P < 0.001) than the DMT. ALT and ALP levels in DM and DMT groups were significantly (P < 0.001) higher than the control; DM was significantly higher (P < 0.001) than DMT.

Conclusion: There was structural and functional hepatic impairment in DM. OG treatment appears to effectively ameliorate these complications including enhancement of bile secretion.

DOI: http://dx.doi.org/10.3126/ajms.v6i3.9960

Asian Journal of Medical Sciences Vol.6(3) 2015 16-21

 

Downloads

Download data is not yet available.
Abstract
862
PDF
994

Author Biography

Esther Olusola Aluko, Department of Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Uyo, Uyo

Department of Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Uyo, Uyo, Nigeria.

Downloads

Published

2014-10-25

How to Cite

Uduak, O. A., Aniekan, P. I., & Aluko, E. O. (2014). Assessment of liver function, bile secretory rate and liver histological changes in type 1 diabetes mellitus rats treated with ocimum gratissimum. Asian Journal of Medical Sciences, 6(3), 16–21. https://doi.org/10.3126/ajms.v6i3.9960

Issue

Vol. 6 No. 3 (2015)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. The journal holds copyright and publishes the work under a Creative Commons CC-BY-NC license that permits use, distribution and reprduction in any medium, provided the original work is properly cited and is not used for commercial purposes. The journal should be recognised as the original publisher of this work.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).