Evaluation of antimalarial and antioxidant activities of the methanol seed extract of Adenanthera pavonina (Linn) in Plasmodium berghei infected mice

Authors

  • Aduragbenro Deborah Adedapo Department of Pharmacology and Therapeutics, College of MedicineUniversity of Ibadan
  • Juliet N Olayinka Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan
  • Oyindamola O. Abiodun Department of Pharmacology and Therapeutics, College of Medicine and Malaria Research Laboratory of the Institute of Advanced Medical Research and Training, (IMRAT), University of Ibadan
  • Ademola A Oyagbemi Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan
  • Odunayo Azeez Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan
  • Adeolu A Adedapo Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan
  • Adegoke A Adeyemi Department of Pharmacognosy, University of Ibadan, Ibadan
  • Jones O Moody Department of Pharmacognosy, University of Ibadan, Ibadan

DOI:

https://doi.org/10.3126/ajms.v5i4.9107

Keywords:

Antimalarial activity, antioxidant, Plasmodium berghei, Adenanthera pavonina

Abstract

Objectives: Malaria and oxidative stress are major health problems in the world in general. The goal of the study is to investigate the antimalarial and antioxidant activities of the methanol seed extract of Adenanthera pavonina linn (ADP) in Plasmodium berghei infected mice.

Methods: Thirty five mice distributed into seven groups of five animals each were used in this study. Plasmodium berghei, was inoculated into Swiss albino mice intraperitoneally with an innoculum size of 1x107 on day zero (D0). The vehicle (1% DMSO), ADP (100, 200, 400, 600 and 800 mg/kg dose) or chloroquine (10 mg/kg) were thereafter administered from D0 – D3. At the end of the antimalarial studies, the blood samples from these animals were collected through cardiac puncture for biochemical assay. The effect of the ADP on the biomakers of oxidative stress was determined in infected mice. In addition in vitro antioxidant activities of ADP were assessed using the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) based assay.

Results: The percentage parasitemia decreased significantly in the parasitized treated group with the crude extract (p < 0.001) compared to the parasitized untreated control group. Also the crude extract, at a dose of 800 mg/kg exerted an antimalarial activity (92.11%) higher than that of chloroquine (88.73%). In the in vitro antioxidant studies, the extract had an IC50> 400 μg/ml which was significantly higher than the standard antioxidant drug, ascorbic acid (IC50 = 1.20 μg/ml). In the case of biochemical and in vivo assay, there was no statistical significant difference (p >0.05) in plasma total protein, malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels in all the treated groups compared to the parasite control group but, there was a statistical significant decrease (p < 0.05) in glutathione (GSH) levels at doses of 400 and 800 mg/kg compared to the parasitized untreated control group.

Conclusions: Methanol seed extract of Adenanthera pavonina demonstrated a significant antimalarial activity but did not exert any antioxidant effect over the parasitized treated mice.

DOI: http://dx.doi.org/10.3126/ajms.v5i4.9107

Asian Journal of Medical Sciences 2014 Vol.5(4); 44-51

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Published

2014-05-16

How to Cite

Adedapo, A. D., Olayinka, J. N., Abiodun, O. O., Oyagbemi, A. A., Azeez, O., Adedapo, A. A., Adeyemi, A. A., & Moody, J. O. (2014). Evaluation of antimalarial and antioxidant activities of the methanol seed extract of Adenanthera pavonina (Linn) in Plasmodium berghei infected mice. Asian Journal of Medical Sciences, 5(4), 44–51. https://doi.org/10.3126/ajms.v5i4.9107

Issue

Vol. 5 No. 4 (2014)

Section

Original Articles

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