Utility of automated cell counter histogram in diagnosis of thrombocytopenia and pseudothrombocytopenia

Authors

DOI:

https://doi.org/10.3126/ajms.v14i7.53334

Keywords:

Ethylenediaminetetraacetic acid; Thrombocytopenia; Pseudothrombocytopenia

Abstract

Background: Automated hematology analyzers have become mainstream of complete blood count (CBC) during the past two decades; they are potential, more accurate to produce results of CBCs. However, often automated analyzers can give false results too, specially, falsely low platelet count due to platelets aggregates, which have to be confirmed on peripheral smears.

Aims and Objectives: The aim and objective of the study was to diagnose thrombocytopenia (TCP) cases and differentiate them from pseudothrombocytopenia (PTCP) cases by analyzing platelet histogram and to determine the sensitivity and specificity of platelet histograms for diagnosing TCP and PTCP.

Materials and Methods: The present study was conducted in the Department of Pathology at Shyam Shah Medical College, Rewa, M.P., after obtaining ethical clearance from the Institutional Ethics Committee. It was prospective study conducted for a period of 15 months from January 2021 to May 2022 on 1000 samples.

Results: The sensitivity and specificity of platelet histogram flags and presence of multiple peaks to diagnose TCP and PTCP cases were calculated as 73.60% and 93.60%, respectively. The present study also interpret that mean platelet volume and platelet distribution width cannot use as indicator to differentiate between TCP and PTCP.

Conclusion: The present study concludes that analysis and interpretation of histogram more specifically platelet histogram flagging provide clue for early detection of PTCP cases prior peripheral smear examination and also helpful in differentiating them from true TCP cases. These platelet histogram flagging can be used as a screening parameter for the detection of PTCP and these are helpful in preventing unnecessary stress for clinician, patients, and their relatives. However, the peripheral smear examination will remain the gold standard to differentiate TCP from, PTCP.

Downloads

Download data is not yet available.
Abstract
72
PDF
129

Author Biography

Rifat Q Usmani, Professor, Department of Pathology, Shyam Shah Medical College, Rewa, Madhya Pradesh, India

Professor Departmenat of Pathology Shyam Shah Medical College Rewa (M.P.)

Downloads

Published

2023-07-01

How to Cite

Hariom Meena, Suresh Kumar Sutrakar, Rifat Q Usmani, Rashmi Jain, Aayushi Guru, Rakesh Gupta, Vinod Kumar Shakya, Parul Singh Rajput, & Shambhavi Sharma. (2023). Utility of automated cell counter histogram in diagnosis of thrombocytopenia and pseudothrombocytopenia. Asian Journal of Medical Sciences, 14(7), 166–170. https://doi.org/10.3126/ajms.v14i7.53334

Issue

Vol. 14 No. 7 (2023)

Section

Original Articles

License

Copyright (c) 2023 Asian Journal of Medical Sciences

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. The journal holds copyright and publishes the work under a Creative Commons CC-BY-NC license that permits use, distribution and reprduction in any medium, provided the original work is properly cited and is not used for commercial purposes. The journal should be recognised as the original publisher of this work.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).