Optimization of preservation solutions to execute testing on cervical smear sample

Authors

DOI:

https://doi.org/10.3126/ajms.v12i6.34299

Keywords:

Cervical cancer, protein preservation solution, HEPES

Abstract

Background: The common methods to preserve cell for protein analyses are in cold condition or treated with freeze solution and packaging in dry ice for shipping. Solution which can preserve cervical cells at room temperature is preferable and cost consuming for laboratory testing.

Aims and Objective: Research and optimized the storage and transport solution for cervical sample which can preserve cells at room temperature for laboratory testing.

Materials and Methods: In this study, cervical specimens were collected in 3 different preservation solutions. Storage and transport of samples was at ambient or refrigerated temperature. The effect of preservation solution and temperature was check by cell visualization under microscope and protein measurement.

Results: Presence of cells were detected in all three solutions. Among those, HEPES solution can preserve the highest number of cells and at room temperature.

Conclusion: HEPES solution appeared suitable to preserve cervical cytology specimens at ambient temperature for further laboratory testing at protein and DNA level.

Downloads

Download data is not yet available.
Abstract
284
PDF
343

Downloads

Published

2021-06-01

How to Cite

Le, Q. T., Pham, T. H., Nguyen, Q. T. C., Truong, Q. P., Nguyen, T. T. T., & Pham, T.-H. (2021). Optimization of preservation solutions to execute testing on cervical smear sample. Asian Journal of Medical Sciences, 12(6), 34–37. https://doi.org/10.3126/ajms.v12i6.34299

Issue

Vol. 12 No. 6 (2021)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. The journal holds copyright and publishes the work under a Creative Commons CC-BY-NC license that permits use, distribution and reprduction in any medium, provided the original work is properly cited and is not used for commercial purposes. The journal should be recognised as the original publisher of this work.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).