Effects of 469 E/K polymorphism of ICAM1 gene in ischemic stroke and its association with stroke severity and outcome

Authors

  • Karthikeyan Pethusamy PhD Scholar, MD(AIIMS), Room No: 3018, Department of Biochemistry, All India Institute of Medical Sciences, New Delhi
  • Ashikh A Seethy PhD Scholar, MD(AIIMS), Room No: 3018, Department of Biochemistry, All India Institute of Medical Sciences, New Delhi
  • Maheshwari Kulandhasamy Assistant Professor, Department of Biochemistry, Maulana Azad Medical College, New Delhi
  • Pinki Garg Associate Professor, Department of Biochemistry, North Delhi Municipal Corporation Medical College, New Delhi
  • Debashish Chowdhury Director, Professor and Head, Department of Neurology, Govind Ballabh Pant Institute of Postgraduate Medical Education and Research, New Delhi
  • Sarita Agarwal Professor and Head, Department of Biochemistry, Hamdard Institute of Medical Sciences and Research, Delhi
  • Arun Kumar Professor and Head, Department of Biochemistry, Jagannath Gupta Institute of Medical Sciences and Hospital, Budge Budge, Kolkata http://orcid.org/0000-0002-8800-0296
  • Ruby Dhar PhD Scientist, Department of Biochemistry, AIIMS, New Delhi, India https://orcid.org/0000-0003-3600-6554
  • Subhradip Karmakar Associate Professor and Team Leader , Department of Biochemistry, AIIMS, New Delhi, India https://orcid.org/0000-0002-4757-8729

DOI:

https://doi.org/10.3126/ajms.v12i1.30985

Keywords:

Ischemic stroke, Intercellular Adhesion Molecule-1, Polymorphism, Polymerase Chain Reaction

Abstract

Background: Stroke is the second leading cause of death globally and it is a major cause of long-term, physical, psychological, and social disability among the elderly. Increasing evidence shows that ischemic injury and inflammation account for its pathogenic progression. So, we studied the association of Intercellular Adhesion Molecule 1 (ICAM1) polymorphism with ischemic stroke, stroke severity, and outcome.

Aims and Objectives: To compare ICAM1 469 E/K polymorphism in ischemic stroke patients with healthy controls, and to study its association with stroke severity and outcome.

Materials and Methods: Fifty patients of ischemic stroke and hundred healthy individuals were included. The stroke severity was assessed clinically and radiologically. Outcome was measured at three and six months of stroke onset. Genomic DNA was used for Allele-Specific PCR to detect ICAM1 469 E/K polymorphism. The subjects were categorized into EE, EK, and KK genotypes.

Results: The odds of EK genotype to develop stroke was 0.41 (95 % CI; 0.17 - 0.92) (p = 0.07) and of KK genotype was 0.41 (95 % CI; 0.11 - 0.87) (p = 0.04) compared to EE genotype. Subjects with ICAM1K allele had significantly reduced risk of stroke compared with those with E allele. (RR: 0.55; 95% CI: 0.35-0.87) (p=0.03).

Conclusion: Subjects with ICAM1K allele had significantly reduced the risk of developing stroke. 469 E/K polymorphism of the ICAM1 gene does not significantly affect stroke severity, mortality, and outcome.

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Published

2021-01-01

How to Cite

Pethusamy, K., Seethy, A. A., Kulandhasamy, M., Garg, P., Chowdhury, D., Agarwal, S., Kumar, A., Dhar, R., & Karmakar, S. (2021). Effects of 469 E/K polymorphism of ICAM1 gene in ischemic stroke and its association with stroke severity and outcome. Asian Journal of Medical Sciences, 12(1), 2–7. https://doi.org/10.3126/ajms.v12i1.30985

Issue

Vol. 12 No. 1 (2021)

Section

Original Articles

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