In vivo antiplasmodial activities of Nauclea latifolia

Authors

  • Ettebong Okon Ette Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo,
  • Edwin P.M. Ubulom Pharmaceutical Microbiology and Parasitology Unit, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Uyo
  • Edet C. Ekpenyong Department of Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Uyo
  • Samuel U. Ekong Pharmaceutical Microbiology and Parasitology Unit, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Uyo
  • Ezekiel O. Akpan Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo
  • Domu V. Tambari Pharmaceutical Microbiology and Parasitology Unit, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Uyo

DOI:

https://doi.org/10.3126/ajms.v6i3.11361

Keywords:

Antiplasmodial, extract, parasitaemia, Plasmodium berghei berghei

Abstract

Background: The plant Nauclea latifolia is widely used in the southern parts of Nigeria for the treatment of malaria. Malaria is the number one killer disease in Nigeria. WHO reports that malaria continues to cause approximately 207 million cases of infection around the world and kills yearly about 627,000 people. It still kills a child somewhere in the world every minute. The study was aimed at evaluating the in vivo antiplasmodial activities of the extract and fractions (n-hexane, chloroform, ethyl acetate, butanol, aqueous) of the stembark in Plasmodium berghei berghei infected mice.

Methods: Through oral administration of the extract (100, 200 and 300mg/kg) and fractions (200mg/kg), antiplasmodial activities were screened using 4-day suppressive, 7-day curative and repository tests. Significance was determined using ANOVA followed by Tukey-Kramer multiple comparison post- test using Graphpad Instat 3.10. A probability level of less than 5% was considered significant.

Results: The extract exhibited significant dose-dependent antiplasmodial activity in the suppressive and repository tests. A significant (p<0.05-0.001) dose-dependent reduction in parasitaemia in extract-treated groups in curative test was also observed. The aqueous fraction had the highest percentage chemosuppressive effect (67.71%).The extract also dose-dependently increased the survival days of the infected mice. The preliminary phytochemical investigation showed the presence of alkaloids, saponins, tannins, flavonoids and cardiac glycosides.

Conclusion: This result confirmed the ethnobotanical use of the plant as malarial remedy and calls for further investigation of its phytochemical components and its antimalarial potentials.

DOI: http://dx.doi.org/10.3126/ajms.v6i3.11361

Asian Journal of Medical Sciences Vol.6(3) 2015 6-11


 

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Author Biography

Domu V. Tambari, Pharmaceutical Microbiology and Parasitology Unit, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Uyo

 

 

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Published

2014-11-01

How to Cite

Ette, E. O., Ubulom, E. P., Ekpenyong, E. C., Ekong, S. U., Akpan, E. O., & Tambari, D. V. (2014). In vivo antiplasmodial activities of Nauclea latifolia. Asian Journal of Medical Sciences, 6(3), 6–11. https://doi.org/10.3126/ajms.v6i3.11361

Issue

Vol. 6 No. 3 (2015)

Section

Original Articles

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