A simple method to detect plant based inhibitors of glycation induced protein cross-linking

Authors

  • HKI Perera University of Peradeniya
  • HASK Ranasinghe University of Peradeniya

DOI:

https://doi.org/10.3126/ajms.v6i1.10181

Keywords:

Glycation, Protein cross-links, SDS-PAGE, Bryophyllum pinnatum, Coriandrum sativum, Murraya koenigi

Abstract

Background: Glycation induced cross-linking of proteins are associated with chronic diabetic complications. Inhibition of protein glycation is one of the therapeutic approaches to prevent the progression of diabetic complications.

Objective: Objective of this study was to establish a simple method to identify medicinal plants which can inhibit glycation induced protein cross-linking.

Methods: Lysozyme was incubated at 37°C up to 4 weeks with different concentrations of glucose, fructose and ribose in sodium phosphate buffer (pH 7.4). Appropriate controls and blanks were carried out. Aminoguanidine (AG) was used as the standard inhibitor. Water extracts of Bryophyllum pinnatum leaves, Coriandrum sativum seed and Murraya koenigi leaves were used as potential inhibitors. Aliquots were removed from the incubation mixtures at intervals and analyzed for the presence of AGE induced protein cross-links, using SDS-PAGE. Appearance and the intensity of high molecular weight products were compared.

Results: Extent of cross-linking was dependent on the sugar concentration. Cross-linking was slowest in the presence of glucose and fastest in the presence of ribose. AG inhibited glycation induced protein cross-linking in the presence of all three sugars. B. pinnatum leaves, C. sativum seeds and M. koenigi leaves inhibited protein cross-linking in the presence of sugar. This inhibition was greater than that of AG.

Conclusions: We have established a simple SDS-PAGE method to identify medicinal plants which inhibit glycation induced protein cross-linking. We also demonstrated the effectiveness of B. pinnatum leaves, C. sativum seed and M. koenigi leaves in inhibiting glycation induced protein cross-linking in vitro.

DOI: http://dx.doi.org/10.3126/ajms.v6i1.10181

Asian Journal of Medical Sciences Vol.6(1) 2015 28-33

Downloads

Download data is not yet available.
Abstract
1045
PDF
1430

Author Biographies

HKI Perera, University of Peradeniya

Department of Biochemistry, Faculty of Medicine,

HASK Ranasinghe, University of Peradeniya

Postgraduate Institute of Science

Downloads

Additional Files

Published

2014-07-24

How to Cite

Perera, H., & Ranasinghe, H. (2014). A simple method to detect plant based inhibitors of glycation induced protein cross-linking. Asian Journal of Medical Sciences, 6(1), 28–33. https://doi.org/10.3126/ajms.v6i1.10181

Issue

Vol. 6 No. 1 (2015)

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. The journal holds copyright and publishes the work under a Creative Commons CC-BY-NC license that permits use, distribution and reprduction in any medium, provided the original work is properly cited and is not used for commercial purposes. The journal should be recognised as the original publisher of this work.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).