<i>In vitro</i> seed germination and seedling development of <i>Phaius tancarvilleae</i> (L’Her.) Blume.

Authors

  • Bijaya Pant Central Department of Botany, T.U., Kirtipur
  • Sumitra Shrestha Central Department of Botany, T.U., Kirtipur
  • Shreeti Pradhan Central Department of Botany, T.U., Kirtipur

DOI:

https://doi.org/10.3126/sw.v9i9.5518

Keywords:

Orchid, Invitro, Protocorm, Media

Abstract

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination.

Keywords: Orchid; Invitro; Protocorm; Media

DOI: http://dx.doi.org/10.3126/sw.v9i9.5518

SW 2011; 9(9): 50-52

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How to Cite

Pant, B., Shrestha, S., & Pradhan, S. (2011). <i>In vitro</i> seed germination and seedling development of <i>Phaius tancarvilleae</i> (L’Her.) Blume. Scientific World, 9(9), 50–52. https://doi.org/10.3126/sw.v9i9.5518

Issue

Vol. 9 No. 9 (2011)

Section

Articles