Simple and Rapid Liquid Chromatography Method for Determination of Rifabutin in Plasma

Authors

  • AK Hemanth Kumar National Institute for Research in Tuberculosis, Chetput, Chennai,
  • V Sudha National Institute for Research in Tuberculosis, Chetput, Chennai,
  • Geetha Ramachandran National Institute for Research in Tuberculosis, Chetput, Chennai,

DOI:

https://doi.org/10.3126/saarctb.v9i2.7975

Keywords:

tuberculosis, rifabutin, liquid chromatography

Abstract

A high performance liquid chromatographic method for determination of rifabutin in human plasma was  developed. The method involved deproteinisation of the sample with acetonitrile and analysis of the  supernatant using a reversed-phase C18 column (250mm) and UV detection at a wavelength of 265nm.  The assay was specific for rifabutin and linear from 0.025 to 10.0μg/ml. The relative standard deviation  of intra- and inter-day assays was lower than 10%. The method was able to remove interfering materials  in plasma, yielding an average recovery of rifabutin from plasma of 101%. Due to its simplicity, the assay  can be used for pharmacokinetic studies of rifabutin.

SAARC Journal of Tuberculosis, Lung Diseases & HIV/AIDS; 2012; IX(2) 26-29

DOI: http://dx.doi.org/10.3126/saarctb.v9i2.7975

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Published

2013-04-23

How to Cite

Hemanth Kumar, A., Sudha, V., & Ramachandran, G. (2013). Simple and Rapid Liquid Chromatography Method for Determination of Rifabutin in Plasma. SAARC Journal of Tuberculosis, Lung Diseases and HIV/AIDS, 9(2), 26–29. https://doi.org/10.3126/saarctb.v9i2.7975

Issue

Vol. 9 No. 2 (2012)

Section

Articles

License

Copyright © SAARC Tuberculosis and HIV/AIDS Centre (STAC), all rights reserved, no part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means without prior permission of the STAC.