Production of Surface Active Glycolipid by Serratia marcescens NSK-1 Isolated from Petroleum Contaminated Soil

Authors

  • C.U. Anyanwu Department of Microbiology, University of Nigeria, Nsukka
  • S.K.C. Obi Department of Microbiology, University of Nigeria, Nsukka
  • B.N. Okolo Department of Microbiology, University of Nigeria, Nsukka

DOI:

https://doi.org/10.3126/on.v8i1.4308

Keywords:

Biosurfactant, bioemulsifier, emulsification, surface tension, Serratia marcescens

Abstract

Biosurfactant-producing Serratia marcescens NSK-1 strain was isolated from soil contaminated with petroleum products. The organism, when grown in mineral salts medium with sucrose as sole carbon source, produced a surface-active anthrone-positive glycolipid. The biosurfactant was partially purified by liquid-liquid partition chromatography in chloroform-methanol mixture (1:1) and silica gel chromatography. It reduced the surface tension of distilled water from 72 dynes/cm to 40 dynes/cm and exhibited good emulsification activity with some vegetable oils and hydrocarbons. The biosurfactant exhibited stable surface activity over a wide range of temperature (30-100°C), pH (2-12) and salt concentration. There was no significant toxic effect shown by the biosurfactant towards mice when tested using the World Health Organization guidelines.

DOI: 10.3126/on.v8i1.4308

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How to Cite

Anyanwu, C., Obi, S., & Okolo, B. (2011). Production of Surface Active Glycolipid by Serratia marcescens NSK-1 Isolated from Petroleum Contaminated Soil. Our Nature, 8(1), 1–11. https://doi.org/10.3126/on.v8i1.4308

Issue

Vol. 8 No. 1 (2010)

Section

Articles

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