Study of Phytochemical, Antioxidant and Antimicrobial Activity of Artocarpus heterophyllus

Authors

  • Nita Thapa Department of Biotechnology, Kathmandu University, Dhulikhel
  • Pratiksha Thapa Department of Biotechnology, Kathmandu University, Dhulikhel
  • Jaya Bhandari Department of Biotechnology, Kathmandu University, Dhulikhel
  • Prasodhan Niraula Department of Biotechnology, Kathmandu University, Dhulikhel
  • Nikita Shrestha Department of Biotechnology, Kathmandu University, Dhulikhel
  • Bhupal G Shrestha Department of Biotechnology, Kathmandu University, Dhulikhel

DOI:

https://doi.org/10.3126/njb.v4i1.16347

Keywords:

Phytochemical, Antioxidant, Antimicrobial, IC50, ZOI (Zone of Inhibition).

Abstract

In today’s world, search for natural medicines is increasing as a result of drug resistance of pathogens and also due to negative consequences of antibiotic. Presence of phytochemicals, antioxidant potential and antimicrobial activity of Artocarpus heterophyllus was carried out in this study. Leaf of this plant was subjected to warm extraction with three different solvents namely methanol, aqueous methanol and ethyl acetate. Leaf extract showed the presence of coumarin, alkaloid, terpenoid in methanol solvent; tannin, coumarin, saponin in aqueous methanol extract and coumarin, terpenoids in ethyl acetate solvent. Further, antimicrobial activity was assessed through disc diffusion method with six pathological bacteria and two fungi strains in four different concentrations of plant extract. Largest ZOI of 16mm was obtained against B. subtilis in 200mg/ml concentration for ethyl acetate extract. Antioxidant potential was measured by DPPH (Diphenyl-2-picrylhydrazyl) assay. DPPH free radical Scavenging Activity was expressed in % inhibition with L Ascorbic acid as standard and leaf extract in methanol showed the best activity.

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Published

2016-12-31

How to Cite

Thapa, N., Thapa, P., Bhandari, J., Niraula, P., Shrestha, N., & Shrestha, B. G. (2016). Study of Phytochemical, Antioxidant and Antimicrobial Activity of Artocarpus heterophyllus. Nepal Journal of Biotechnology, 4(1), 47–53. https://doi.org/10.3126/njb.v4i1.16347

Issue

Vol. 4 No. 1 (2016)

Section

Original Research Articles

License

Copyright (c) 2016 Nepal Journal of Biotechnology

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