Dengue Virus Detection by Serological and Molecular Method in Different Hospitals of Nepal

Authors

  • Yogendra Shah Everest International Clinic and Research Center Pvt. Ltd, Kalanki, Kathmandu
  • Govind Prasad Gupta Everest International Clinic and Research Center Pvt. Ltd, Kalanki, Kathmandu
  • Kishor Pandey Everest International Clinic and Research Center Pvt. Ltd, Kalanki, Kathmandu
  • Sher Bahadur Pun Everest International Clinic and Research Center Pvt. Ltd, Kalanki, Kathmandu
  • Krishna Prasad Pant Department of Microbiology, Siddhanath Science Campus Mahendranagar, Kanchanpur
  • Santosh Dhakal National Zoonoses and Food Hygiene Research Center, Chagal, Kathmandu
  • Basu Dev Pandey Everest International Clinic and Research Center, Kathmandu

DOI:

https://doi.org/10.3126/mjsbh.v11i2.7905

Keywords:

dengue, IgM capture ELISA, RT-PCR

Abstract

Introduction: Dengue is an emerging mosquito-borne viral disease in the world and is the serious public health problem of Nepal.

Methods: This study was designed to determine sero-epidemiology of dengue virus infection during the period (June-Nov) of 2010 among suspected patients with fever visiting Koshi Zonal Hospital (KZH), Biratnagar, Narayani sub-regional Hospital (NSH), Birgunj, Sukraraj Tropical and Infectious Disease Hospital (STIDH), Kathmandu and Dhading District Hospital (DDH), Dhadingbeshi. The sero-prevalence of anti-dengue IgM antibody was determined by enzyme linked immunosorbent assay (ELISA).

Results: Among 271 serum samples tested, the anti-dengue IgM positivity was 14.4%. Sero-positivity in male was 10.7% of total and that in female was 3.7%.  Among different age groups, the highest positive cases 11.8% were from age group 15-50 years and found least among the age group above 50 years 0.4%. Out of 4 different hospitals, the highest positive positive cases from STIDH with 9.2% and the least positive cases were from DDH (0.4%). RT-PCR showed 4.7% positivity of 21 samples tested.

Conclusions: Enzyme immunoassay and RT-PCR serological marker can be used to diagnose the acute patients of dengue during outbreaks.

Medical Journal of Shree Birendra Hospital; July-December 2012/vol.11/Issue2/24-27

DOI: http://dx.doi.org/10.3126/mjsbh.v11i2.7905

 

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Published

2013-04-05

How to Cite

Shah, Y., Gupta, G. P., Pandey, K., Pun, S. B., Pant, K. P., Dhakal, S., & Pandey, B. D. (2013). Dengue Virus Detection by Serological and Molecular Method in Different Hospitals of Nepal. Medical Journal of Shree Birendra Hospital, 11(2), 24–28. https://doi.org/10.3126/mjsbh.v11i2.7905

Issue

Vol. 11 No. 2 (2012)

Section

Original Articles

License

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