Chemical and biological analysis of extracts of Acorus calamus L.

Authors

  • Aaradhana Pokharel Department of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, Nepal
  • Arjun Thapa Department of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, Nepal
  • Homa Karki Department of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, Nepal
  • Rakesh K Yadav Department of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, Nepal
  • Samjhana Bharati Department of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, Nepal
  • Timila Shrestha Department of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, Nepal
  • Puspa Lal Homagai Department of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, Nepal
  • Binita Maharjan Department of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, Nepal
  • Ram Lal Shrestha Department of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu, Nepal

DOI:

https://doi.org/10.3126/jncs.v43i2.53813

Keywords:

Acorus calamus, GC-MS, TPC, TFC, Antibacteria, Antioxidant, Cytotoxicity

Abstract

The powdered plant materials of Acorus calamus were subjected to successive extraction using the cold percolation method with methanol, hexane, and chloroform solvent. Qualitative phytochemical analysis of methanol, hexane, and chloroform extracts showed the presence of alkaloids, saponins, glycosides, sterols, triterpenoids, and carbohydrates. Five different major compounds were identified by GC-MS analysis of the chloroform extract, with isoprothiolane (83.11%) being the most prevalent. The total phenolic content in the chloroform extract was calculated at 17.39 mg Gallic acid equivalent /g and the total flavonoid content was 3.37 mg quercetin equivalent/g of dry extract. The IC50 value of chloroform extract was found to be 576.19 μg/mL, and the LC50 value was found to be 66.21 μg/mL. Antibacterial activity was shown in Staphylococcus aureus in chloroform extract with a ZOI of 7 mm.

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Published

2023-04-25

How to Cite

Pokharel, A., Thapa, A., Karki, H., Yadav, R. K., Bharati, S., Shrestha, T., Homagai, P. L., Maharjan, B., & Shrestha, R. L. (2023). Chemical and biological analysis of extracts of Acorus calamus L. Journal of Nepal Chemical Society, 43(2), 151–158. https://doi.org/10.3126/jncs.v43i2.53813

Issue

Vol. 43 No. 2 (2023)

Section

Research Article

License

© Journal of Nepal Chemical Society