Characterization of Heat Shock Protein (HSP 70) Sequence in Leishmania donovani

Authors

  • Mahendra Maharjan Central Department of Zoology, TU, Kirtipur and School of Life Sciences, Jawaharlal Nehru University, New Delhi

DOI:

https://doi.org/10.3126/jist.v20i1.13914

Keywords:

Leishmania donovani, HSP 70, cloning, sequencign, cytoplasmic protein

Abstract

Heat shock protein HSP70 is the highly conserved protein along the kinetoplastid phylogenetic spectrum. A full length hsp70 gene was amplified, cloned and sequenced. The protein sequence was aligned and phylogentic tree was constructed. The Leishmania donovani HSP70 protein sequence (653 amino acids) is slightly larger than the Human HSP70 protein sequence (641 amino acids) with 72% identity. The putative protein sequence is 98%, 97%, 96% and 92% identical to L. major, L. infantum, L. tarentolae and L. braziliensis respectively. Evolutionary relationship showed that kinetoplastid parasites are closely related to the human compared to prokaryotes, E. coli.

Journal of Institute of Science and Technology, 2015, 20(1): 82-86

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Published

2015-11-25

How to Cite

Maharjan, M. (2015). Characterization of Heat Shock Protein (HSP 70) Sequence in Leishmania donovani. Journal of Institute of Science and Technology, 20(1), 82–86. https://doi.org/10.3126/jist.v20i1.13914

Issue

Vol. 20 No. 1 (2015)

Section

Research Articles

License

The views and interpretations in this journal are those of the author(s). They are not attributable to the Institute of Science and Technology, T.U. and do not imply the expression of any opinion concerning the legal status of any country, territory, city, area of its authorities, or concerning the delimitation of its frontiers of boundaries.

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