Amplified Fragment Length Polymorphism Mapping of Tomato Backcross Populations

Abstract

Tomato backcross populations of Lycopersicon exculentum cv. Money Maker x Lycopersicon pennellii LA716 were used for construction of an Amplified Fragment Length Polymorphism (AELP) map based on pennellii markers. Ten primer combinations (7 of Eco-Mse and 3 of Pst-Mse) were used. This map spanned 893.7 cM and contained 187 AFLP markers, consisting 130 previously found markers and 57 new markers. A total of 141 markers were found from Eco-Mse primer combination and 46 from Pst-Mse primer combination. The Eco-Mse AFLP markers were not evenly distributed over the chromosomes. The Pst-Mse AFLP markers were found quite frequently and evenly distributed in the more distal part of the chromosomes. Eco-Mse markers were found quite more in numbers in chromosomes 5 and 9, whereas Pst-Mse markers in chromosomes 1 and 7. In chromosomes 4, 6, 7 and 8, the new markers and previously found markers were found quite equal in numbers. The average number of informative markers per primer combination was 18.7 ranging from 6 (E33M47) to 36(E35M48). The average size of map per chromosome was found 74.5 cM ranging from 46.2 cM of chromosome 8 and 105.5 cm of chromosome 3. Fourteen BC₁ plants for BC₂ and subsequently 12 BC₂ plants for BC₃ generation were selected to obtain inbred lines with genes of interest. BC₁ plants were selected each representing about 50% Lycopersicon pennellii LA716 genome and BC₂ plants were selected each representing about 25% genome coverage, and together cover 100% of the Lycopersicon pennellii genome based on scored markers

J. Inst. Agric. Anim. Sci. 2003 24:21-28.