HPLC analysis of biomarkers of Toluene and Xylene in human urine samples

Authors

DOI:

https://doi.org/10.3126/ijosh.v13i3.46615

Keywords:

Hippuric acid, Methylhippuric acid, Biomarker, HPLC, Method validation

Abstract

Introduction: Urinary Hippuric acid (HA) and Methylhippuric acid (MHA) are considered reliable biomarkers for monitoring exposure to toluene and xylenes. A simple and robust HPLC method for simultaneous determination of HA and MHA in urine samples was developed and validated as per Internation Conference on Harmonization (ICH) Q2 guidelines.

Methods: Mobile phase consisting of two solvents A & B, solvent A was 0.1% TFA in water, and solvent B was 0.1% TFA in acetonitrile.  Separation was performed in gradient elution mode and the time program was as follows - Time (min)/%B: 0/20, 4/20, 11/80, 11.1/90, 16/90, 16.1/20, 25/20. The detection wavelength was 205 nm.

Results: The limits of detection (LOD) and quantification (LOQ) of HA in urine were 0.3 and 1.0 mg/L, and for MHA were 0.25 mg/L and 1.0 mg/L respectively and the recovery for HA and MHA were 95% and 98.9% respectively. 

Conclusion: The developed method was successfully applied for the analysis of urine samples.

Downloads

Download data is not yet available.
Abstract
325
pdf
140

Author Biographies

Pawan Kumar Maurya, ICMR-Centre for Ageing & Mental Health, Kolkata, India

(Corresponding author)

Scientist-C, ICMR-Centre for Ageing & Mental Health (I-CAM), Block DP-1, Sector V, Salt Lake, Kolkata-700091, India
E-mails: maurya.pk@icmr.gov.in, maurya_pawan@yahoo.com

Anupa Yadav,  ICMR-Centre for Ageing & Mental Health (I-CAM), Kolkata-700091, India

Technical Officer, ICMR-Centre for Ageing & Mental Health (I-CAM), Block DP-1, Sector V, Salt Lake, Kolkata-700091, India
E-mails; anupayadav08@gmail.com, yadav.a@gov.in

Pankaj Maurya, Institute of Biosciences and Biotechnolgy, Chatrapati Sahu Ji Maharaj University, Kanpur, India

M.Sc. Student, Department of Environmental Sciences, Institute of Biosciences and Biotechnolgy, Chatrapati Sahu Ji Maharaj University, Kanpur-208024, India
E-mail: environmentalistpm@gmail.com

Downloads

Published

2023-07-04

How to Cite

Maurya, P. K., Yadav, A., & Maurya, P. (2023). HPLC analysis of biomarkers of Toluene and Xylene in human urine samples. International Journal of Occupational Safety and Health, 13(3), 331–337. https://doi.org/10.3126/ijosh.v13i3.46615

Issue

Vol. 13 No. 3 (2023)

Section

Original Articles

License

Copyright (c) 2023 Pawan Kumar Maurya, Anupa Yadav, Pankaj Maurya

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

This license enables reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.