Analysis of Phytoconstiuents and Cytotoxic Activities of Different Parts of Ocimum sanctum

Abstract

Phytochemical screening of extracts of Ocimum Sanctum revealed the presence of fatty acids, steroids, triterpenoid, phenolic compound, flavonoid, glycoside, quinines, and carotenoids. β-sitosterol and Ursolic acid were isolated from leaves of the plant by using column chromatography and  identification  of these compounds were performed with the help of melting points, Co-TLC  and spectroscopic techniques such as IR, ¹HNMR and ¹³ CMR. GC-MS of hexane fraction showed presence of pentanal, sotolone, hexane-3-one, hexane-2-ol, caryphyllene, benzene 1,2 di-carbolylic acids, isoeugenol, cavracrol and eugenol compounds. The brine shrimp bio assay showed  LC₅₀ value 213.7,  97.72 and 21.87 for hexane, ethyl acetate and methanol extracts of leaves respectively, where as LC₅₀ value 169.84, 141.25 and 151.35 for hexane, ethyl acetate and methanol  extracts of stem respectively. All extracts are pharmacologically active.

DOI: http://dx.doi.org/10.3126/ijasbt.v1i3.8609

Int J Appl Sci Biotechnol, Vol. 1(3) 2013 : 137-144