Genomic DNA Extraction Protocol for Artemisia Annua L. Without Using Liquid Nitrogen and Phenol

Authors

  • Ankit Agrawal MP Council of Science and Technology, Vigyan Bhawan, Nehru Nagar, Bhopal (M.P.) http://orcid.org/0000-0003-0427-1126
  • Anjana Sharma Bacteriology laboratory, Department of Post Graduate Studies and Research in Biological Sciences, R. D. University, Jabalpur (M.P.)
  • Narmada Prasad Shukla MP Council of Science and Technology, Vigyan Bhawan, Nehru Nagar, Bhopal (M.P.) and MP Pollution Control Board, E-5, Arera Colony, Bhopal (M.P.)

DOI:

https://doi.org/10.3126/ijasbt.v4i4.15788

Keywords:

Cetyl Trimethyl Ammonium Bromide (CTAB), DNA Extraction, Artemisia annua, artemisinin, Malaria

Abstract

Artemisia annua is an important medicinal plant, used for curing various diseases especially malaria. It secretes verities of secondary metabolites which hinders in the DNA extraction. This investigation describes an efficient DNA extraction protocol for A. annua based upon the Cetyl Trimethyl Ammonium Bromide (CTAB) extraction method without using hazardous chemicals i.e. liquid nitrogen and phenol. The developed protocol is simple, reliable and operative in normal laboratory condition yielding intact DNA having good quantity (502.7 to 1288.5 ng/µl) and quality (A260/280 ratio - 1.82 to 1.85) in two working days. Proper amplification of extracted DNA indicates its suitability for the various molecular biological applications. 

Int J Appl Sci Biotechnol, Vol 4(4): 448-451 

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Published

2017-01-06

How to Cite

Agrawal, A., Sharma, A., & Shukla, N. P. (2017). Genomic DNA Extraction Protocol for Artemisia Annua L. Without Using Liquid Nitrogen and Phenol. International Journal of Applied Sciences and Biotechnology, 4(4), 448–451. https://doi.org/10.3126/ijasbt.v4i4.15788

Issue

Vol. 4 No. 4 (2016)

Section

Research Articles: Biological Sciences