In Vitro Callus Proliferation from Leaf Explants of Green Gram after In Situ UV-B Exposure

Authors

  • K Rajendiran Department of Botany, K.M. Centre for Post Graduate Studies, Pondicherry - 605 008 http://orcid.org/0000-0002-5127-3207
  • S Vidya Department of Botany, K.M. Centre for Post Graduate Studies, Pondicherry - 605 008
  • D Arulmozhi Department of Botany, K.M. Centre for Post Graduate Studies, Pondicherry - 605 008

DOI:

https://doi.org/10.3126/ijasbt.v4i1.14719

Keywords:

Callus proliferation, Green gram, Leaf explants, Three varieties, Ultraviolet-B,

Abstract

Callus induction was tried with leaf explants (third leaf from top of canopy) harvested from in situ control and supplementary UV-B irradiated (UV-B = 2 hours daily @ 12.2 kJ m-2 d-1; ambient = 10 kJ m-2 d-1) three varieties of green gram viz. CO-8, NVL-585 and VAMBAN-2 to study their viability for germplam conservation. Callus induction occurred both in control and UV-B stressed NVL-585 leaf explants. VAMBAN-2 both in unstressed and UV-B stressed conditions did not initiate callus. Only control leaf explants from CO-8 proliferated callus. Callus of UV-B irradiated NVL-585 weighed less (51.28 %) than control. Parenchyma cells were smaller in callus inducted from in situ UV-B exposed NVL-585 leaf explants. The leaf explants from UV-B stressed NVL-585 varieties of green gram responded to in vitro callus proliferation making them fit for germplasm conservation for cultivating in UV-B elevated environment.

Int J ApplSci Biotechnol, Vol 4(1): 82-91

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Published

2016-03-31

How to Cite

Rajendiran, K., Vidya, S., & Arulmozhi, D. (2016). In Vitro Callus Proliferation from Leaf Explants of Green Gram after In Situ UV-B Exposure. International Journal of Applied Sciences and Biotechnology, 4(1), 82–91. https://doi.org/10.3126/ijasbt.v4i1.14719

Issue

Vol. 4 No. 1 (2016)

Section

Research Articles: Biological Sciences